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Quick Overview
10 x 96 Well
1-5 mL
1-5 mL
Details
The 10 x 96 well plasmid kit was designed for high-throughput purification of plasmid DNA from 1 – 5 ml of cultured bacterial cells per/well. The kit utilizes a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. This kit utilizes a centrifuge capable of running 96-well plates.
Typical yields are 20 – 35 µg for high-copy number plasmid and 3 – 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions.
Features:
• 10 x 96 Well Plasmid kit
• Centrifuge version 1 – 5 mL cultured bacterial cells
• Filter plates included
Typical yields are 20 – 35 µg for high-copy number plasmid and 3 – 10 µg for low-copy number plasmid from 4 ml of cultured bacterial cells (E. coli). The purified DNA is ready for use in restriction enzyme digestion, ligation, PCR and sequencing reactions.
Features:
• 10 x 96 Well Plasmid kit
• Centrifuge version 1 – 5 mL cultured bacterial cells
• Filter plates included
Additional Information
| Applications | Plasmid Isolation |
|---|---|
| Nucleic Acid Binding Capacity | N/A |
| Nucleic Acid | Plasmid |
| Number of Preps | N/A |
| Protocol Time | N/A |
| Sample Type | Bacterial Culture |
| Target Length | N/A |
| Yield | Variable |
410 Pierce Street, Houston, TX 77002
www.sycamorelifesciences.com
Business Hours: 8:30 am to 5:30 pm Central Time