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Eurogentec Takyon™ Low ROX SYBR® 2X Mastermix Blue, 5 x 7.5 ml
Quick Overview
DNA Target
SYBR Green
Low ROX Included
3750 rxn
Details
Takyon™ Low ROX SYBR® 2X MasterMix dTTP Blue is a convenient, optimized mixture of premeasured ingredients in a ready-to-use format for the performance of real-time PCR detection of universal DNA targets using the fluorescent intercalating dye SYBR® Green I. Obtain consistent and sensitive results under Fast or regular cycling conditions. MasterMix is formulated with a chemically modified hot-start 3rd generation Phusion DNA polymerase that is activated in just three minutes at 95°C. Incorporates ROX passive reference dye to permit sample fluorescence normalization and also an inert Blue dye for MasterMix dispensing verification. Refer to Technical Data Sheet for kit components and cycling conditions. Compatible with ABI Prism® 7500, QuantStudio™ & ViiA™ and Stratagene Mx4000, Mx3000P & Mx3005P. Can be combined with Takyon™ One-Step Kit Converter to turn MasterMix into a one-step qRT-PCR formulation.
Additional Information
Applications |
qPCR |
RT System Compatibility |
N/A |
Detection Chemistry |
SYBR Green |
dUTP |
No |
Fast Protocol Compatible |
Yes |
Passive Reference Dye |
Low ROX |
Hot Start |
Yes |
Concentration |
2X |
Multiplex Capable |
No |
Number of Reactions |
3750 |
Kit Volume |
5 x 7.5 ml |
Reaction Volume (µL) |
20 µL |
Target |
DNA |
UNG Included |
No |