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Quick Overview
RNA Target
Probe-Based Detection
ROX Included
150 rxn
Probe-Based Detection
ROX Included
150 rxn
Details
Takyon™ One-Step ROX Probe 5X MasterMix dTTP is a convenient, optimized 5X concentrated mixture of premeasured ingredients in a ready-to-use format for the performance of one-step qRT-PCR from total or purified mRNA samples using fluorescent, gene-specific probe molecules. Obtain consistent and sensitive results under Fast or regular cycling conditions. In a single pipetting step, add EuroScript II MMLV Reverse Transcriptase and RNase inhibitor to MasterMix formulated with a chemically modified hot-start 3rd generation Phusion DNA polymerase (activated in just three minutes at 95°C). 5X concentrated reaction buffer offers the advantage of more dead volume for DNA template. Incorporates ROX passive reference dye to permit sample fluorescence normalization. Refer to Technical Data Sheet for kit components and cycling conditions. Compatible with ABI Prism® 7000, 7300, 7700 & 7900 and ABI StepOne™ & StepOnePlus™.
Additional Information
| Applications | 1-Step qRT-PCR |
|---|---|
| RT System Compatibility | N/A |
| Detection Chemistry | Probe |
| dUTP | No |
| Fast Protocol Compatible | Yes |
| Passive Reference Dye | ROX |
| Hot Start | Yes |
| Concentration | 5X |
| Multiplex Capable | Yes |
| Number of Reactions | 150 |
| Kit Volume | 1.5 ml |
| Reaction Volume (µL) | 20 µL |
| Target | RNA |
| UNG Included | No |
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