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Eurogentec Takyon™ Probe 2X qPCR Mastermix UNG, 5 x 7.5 ml, No ROX
Quick Overview
DNA Target
Probe-Based Detection
No ROX
Multiplex Capable
3750 rxn
Details
Takyon™ No ROX Probe 2X MasterMix UNG is a convenient, optimized mixture of premeasured ingredients in a ready-to-use format for the performance of real-time PCR detection of universal DNA targets using fluorescent, gene-specific probe molecules. Obtain consistent and sensitive results under Fast or regular cycling conditions. MasterMix is formulated with a chemically modified hot-start 3rd generation Phusion DNA polymerase that is activated in just three minutes at 95°C. Refer to Technical Data Sheet for kit components and cycling conditions. Includes dUTP/UNG blend in the reaction buffer to prevent amplification of carry-over contamination from previous PCRs. Suitable for all platforms that do not require a passive reference dye, including Roche LC96 & LC480, Rotor-Gene® 2000, 3000, 6000 & Q, Stratagene Mx4000, Mx3000P & Mx3005P, Bio-Rad CFX96™ & CFX384™, DNA Engine Opticon® 1 & 2 , Chromo4™ & MiniOpticon™, Eppendorf MasterCycler® ep realplex , Cepheid SmartCycler® 1 & 2 , Techne Quantica® and Swift™ Spectrum. Can be combined with Takyon™ One-Step Kit Converter to turn MasterMix into a one-step qRT-PCR formulation.
Additional Information
Applications |
qPCR |
RT System Compatibility |
N/A |
Detection Chemistry |
Probe |
dUTP |
No |
Fast Protocol Compatible |
Yes |
Passive Reference Dye |
None |
Hot Start |
Yes |
Concentration |
2X |
Multiplex Capable |
Yes |
Number of Reactions |
3750 |
Kit Volume |
5 x 7.5 ml |
Reaction Volume (µL) |
20 µL |
Target |
DNA |
UNG Included |
Yes |